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Gel Electrophoresis Restriction Enzymes
554 wordsDNA, Deoxyribonucleic acid, is a double stranded, helical nucleic acid molecule which determines inherited structure of a protein. The steps are made of bases: adenine, guanine, cytosine, and thymine. The sides are sugar and phosphate molecules. Restriction enzymes are enzymes that cut DNA at restriction sites, leaving fragments blunt or sticky. The restriction fragments are separated using a technique called DNA has a negative charge so when an electrical charge is applied it makes DNA move to ...
Free research essays on topics related to: gel electrophoresis, dna fragments, restriction enzymes, power supply, figure 1 -
Dna Gel Electrophoresis Research
1,848 words... ting smaller fragments that travel longer distances than the single cut or uncut, during electrophoresis. Which in turn proves that segment sizes are proportional to distance migrated. On a standard curve, the distance migrated is used in order to extrapolate the size of the DNA (base pairs). For an undigested or uncut p KAN plasmid it is expected that one fragment will be shown, unless the plasmid has been nicked and then will therefore show three. The size of this fragment should be close ...
Free research essays on topics related to: base pairs, uv light, p amp, gel electrophoresis, dna fragments
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