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Gel Electrophoresis Restriction Enzymes
554 wordsDNA, Deoxyribonucleic acid, is a double stranded, helical nucleic acid molecule which determines inherited structure of a protein. The steps are made of bases: adenine, guanine, cytosine, and thymine. The sides are sugar and phosphate molecules. Restriction enzymes are enzymes that cut DNA at restriction sites, leaving fragments blunt or sticky. The restriction fragments are separated using a technique called DNA has a negative charge so when an electrical charge is applied it makes DNA move to ...
Free research essays on topics related to: gel electrophoresis, dna fragments, restriction enzymes, power supply, figure 1 -
Gel Electrophoresis Chain Reaction
892 wordsHuntington Disease, (HD), is a hereditary degenerative brain disease. Usually begining in mid-life, cells in the caudate nucleus of the brain begin to die, causing a relentless deterioration of intellectual ability, emotional control, motor skills, balance and speech. Chorea, or involuntary movements, is nearly always a symptom as well. HD is caused by a single dominant gene. Every child of an affected parent has a 50 % chance of inheriting the gene that causes HD. The HD gene is found on chromo...
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Dna Gel Electrophoresis Research
1,724 wordsThe main objective of this lab was to identify unknown plasmids by observation of their genotype and phenotype. We observed the plasmids phenotype by using it to transform bacteria. When the plasmid is in a bacterial host the antibiotic resistance gene can be expressed and impart resistance to the host. We also analyzed the genotype of the unknown plasmid by performing a variety of molecular genetic techniques such as predigestion, electrophoresis in arose gel, staining with ethidium bromide, an...
Free research essays on topics related to: bone marrow, gel electrophoresis, dna molecule, gene therapy, antibiotic resistance -
Dna Gel Electrophoresis Research
1,848 words... ting smaller fragments that travel longer distances than the single cut or uncut, during electrophoresis. Which in turn proves that segment sizes are proportional to distance migrated. On a standard curve, the distance migrated is used in order to extrapolate the size of the DNA (base pairs). For an undigested or uncut p KAN plasmid it is expected that one fragment will be shown, unless the plasmid has been nicked and then will therefore show three. The size of this fragment should be close ...
Free research essays on topics related to: base pairs, uv light, p amp, gel electrophoresis, dna fragments -
Gel Electrophoresis Carbon Dioxide
2,361 wordsPyruvate Decarboxylase There are two functions of the pyruvate decarboxylase. Pyruvate and hydroxythyl converge together while carbon dioxide molecules are eliminated. Than, hydroxy ethyl group transfers and attaches to TPP to the lipo amide from the E 2 component of the complex. The final transfer brings the molecule back to its original form. (Function) The molecular weight of the enzyme was found to be 240, 000 by polyacrylamide gel electrophoresis (Oba). There are 548 amino acids and very si...
Free research essays on topics related to: gel electrophoresis, lactic acid, carbon dioxide, acid, enzyme
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