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Petri Dishes Bacteria Cells
1,566 wordsIn this lab we observed the growth of bacteria in a conjugation and transformation experiment. We expected the mating plate of the conjunction to grow and the bacteria on the transformation plates to grow also. We also practiced using aseptic techniques to clean the lab benches and to keep the bacteria from being contaminated from outside bacteria. This lab will take a total of 2 days because of the observations that need to be made. The parts of this paper will explain the procedure and the obs...
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Rate Of Reaction Reaction Rate
1,069 wordsThe purpose of this experiment is to measure the rates of reaction of the enzyme Alkaline Phosphatase with the substrate p-nitro phenol phosphate under varying conditions. The concentration of both substrate and enzyme were diluted and the inhibitor vanadate was utilized to determine whether or not the reaction is substrate or enzyme dependent and to see what type of inhibition vanadate was involved. A class of proteins called enzymes catalyzes almost every chemical reaction in a cell. Enzymes i...
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Lab Manual E Coli
1,139 wordsGene Regulation Using the Lac Operon Abstract Gene regulation in cells has been studied extensively in both eukaryotic and prokaryotic organisms. Gene control is employed as a way of saving the cell energy by only producing those enzymes it needs. In eukaryotes, genes are regulated so as maintain levels of cell specificity. In this experiment, the restriction of the production of the -galactosidase enzyme in the prokaryote Escherichia coli can be achieved by employing the lac operon. This operon...
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Autosomal Recessive Lab Manual
1,318 wordsMapping Unknown Mutations Of Mutant Strain U 5914 Mapping Unknown Mutations Of Mutant Strain U 5914 D Abstract Unknown mutant strain U 5914 Drosophila melanogaster with 3 mutated phenotypes: white eyed, dark body color, and incomplete longitudinal vein II and IV. This mutant strain was crossed with wild-type strain and marker strains to study the genetic characteristics of these 3 mutant genes. The results showed that dark body color is an autosomal recessive mutation (db) located on chromosome ...
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Heavy Metals Metal Ions
2,146 wordsThe inhibitory effects of a mercury compound, silver nitrate, and copper sulfate on Bacillus cereus, Escherichia coli, Micrococcus lotus, and Pseudomonas putin Introduction Certain heavy metals can have harmful or inhibitory effects on microorganisms, a process known as oligo dynamic action; for example, some heavy metal ions accumulate in cellular proteins leading to protein denaturation and ultimately cell death (Biology 108 lab manual 2001). A consequence of this is that heavy metals can affe...
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