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Purpose: The purpose of this lab was to gain the quantitative measure of the color difference between Transmitance and absorbance in 8 different samples of water containing varying amounts of iron in a range between 5 - 100 ppm, so that we know which samples where except able for drinking. Data: The Mystery Solution had a transmittance of 6 Calculations 80 ppm (25 ml) = 20 ml 100 ppm This means that in order to get 25 ml of 80 ppm solution 20 ml of water must be added to the previous solution. Absorption: y = 2 -Log (concentration) Y = 2 -Log (10. 3) Y = 0. 98 Mystery Solution: Absorbance = . 0103 X concentration of mystery solution Absorbance = . 05011 Mystery Solution: . 206209615 = . 0103 concentration of mystery solution Concentration = 20 Question 2. 125 / 20 = 6. 25 x Less Question 3. 8 ounces (1 quart/ 32 ounces) (1 liter/ 1. 057 quarts) = . 2365 Discussion: The Spectrophotometer measures the absorbance of light, a beam of light is passed through a transparent tube containing the absorbing liquid. The intensity of light reaching a detector is compared to the intensity reaching the detector in the absence of the absorbing material.
Beer? s law is that the absorbance (defined as log) is proportional to the concentration.
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Research essay sample on 20 Ml Y 2